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| Reactivity: | M,R & M,R |
| Applications: | WB & IHC |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | MCM4 rabbit polyclonal |
Gene Name: | DNA replication licensing factor MCM4 |
Synonyms: | CDC21 homolog, P1-CDC21, Mcm4, Cdc21, Mcmd4, CDC54, NKGCD, NKCD, Minichromosome maintenance 4, Homolog of S. pombe cell devision cycle 21, hCdc21 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse MCM4 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | |
Predicted MW. | 97 kDa |
Observed MW. | 97 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Mouse, Rat | 1: 2000-1: 4000 | lymph node |
IHC | Mouse, Rat | 1: 1200-1: 2400 | testis |
Background
DNA replication licensing factor MCM4 (MCM4), also named Cdc21, acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.This antibody is a rabbit polyclonal antibody raised against the C-terminal 350 aa sequence of MCM4 protein.
Images
| Western blot analysis of MCM4 (GB113065) at dilution of 1: 4000 |
| Immunohistochemistry analysis of paraffin-embedded mouse testis using MCM4 (GB113065) at dilution of 1: 2400 |
| Immunohistochemistry analysis of paraffin-embedded rat testis using MCM4 (GB113065) at dilution of 1: 2400 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
