Your shopping cart is empty!
| Reactivity: | H |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | MCP1 rabbit polyclonal |
Gene Name: | C-C motif chemokine 2 |
Synonyms: | CCL2, GDCF-2, HC11, HSMCR30, MCAF, MCP1, SCYA2, SMC-CF, C-C motif chemokine ligand 2, MCP-1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human MCP1 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 11 kDa |
Observed MW. | 11 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human | 1: 500-1: 1000 | THP-1 |
Background
Monocyte chemotactic protein-1 (MCP-1), also known as CCL2, monocyte chemotactic activating factor (MCAF) or glioma-derived chemotactic factor-2 (GDCF-2), is the product of the human JE gene and a member of the family of C-C (or β) chemokines . The predicted molecular weight of MCP-1 protein is 11-13 kDa, but it may migrate at 20-30 kDa due to glycosylation. MCP-1 is secreted by a variety of cell types in response to pro-inflammatory stimuli and was originally described for its chemotactic activity on monocytes. This activity has led to studies demonstrating its role in diseases characterized by monocyte infiltrates such as psoriasis , rheumatoid arthritis and atherosclerosis . MCP-1 may also contribute to tumor progression and angiogenesis . Signaling by MCP-1 is mediated by the G-protein coupled receptor CCR2 .
Images
 | Western blot analysis of MCP1 (GB113239) at dilution of 1: 1000 Lane 1: THP-1 untreated Lane 2: THP-1 treated with 80nM TPA for 24h Lane 3: THP-1 treated with 80nM TPA for 24h, then treated with 100ng/ml lipopolysaccharide (LPS) for 6h, then with 300ng/ml Brefeldin A added after 3 hours, whole cell lysate |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
