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| Reactivity: | M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Enoyl-[acyl-carrier-protein] reductase, mitochondrial |
Synonyms: | AI195831, Mecr, Nrbf1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse MECR |
Uniprot ID: | Q9DCS3 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 40 kDa / 43 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 2000-1: 3000 | brain |
Background
Catalyzes the NADPH-dependent reduction of trans-2-enoyl thioesters in mitochondrial fatty acid synthesis (fatty acid synthesis type II). Fatty acid chain elongation in mitochondria uses acyl carrier protein (ACP) as an acyl group carrier, but the enzyme accepts both ACP and CoA thioesters as substrates in vitro. Displays a preference for medium-chain over short- and long-chain substrates (By similarity). May provide the octanoyl chain used for lipoic acid biosynthesis, regulating protein lipoylation and mitochondrial respiratory activity particularly in Purkinje cells.
Images
| Western blot analysis of MECR (GB115061) at dilution of 1: 200 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
