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| Reactivity: | H,M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Mediator of RNA polymerase II transcription subunit 8 |
Synonyms: | Activator-recruited cofactor 32 kDa component, ARC32, Mediator complex subunit 8, Med8, MNCb-2386 |
Immunogen: | Recombinant protein corresponding to Mouse MED8 |
Uniprot ID: | Q9D7W5 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. / Observed MW. | 29 kDa / 29 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | liver, testis |
IHC/IF | Mouse, Rat | 1: 600-1: 1200 | brain, testis |
Background
MED8, a component of the Mediator complex, is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. It plays a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins. MED8 has three isoforms with MW 19 kDa, 29 kDa and 33 kDa. And the ubiquitination form will be 38-43 kDa.
Images
| Western blot analysis of MED8 (GB111804) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraffin embedded rat brain using MED8 (GB111804) at dilution of 1: 600 |
| Immunofluorescent analysis of paraffin embedded mouse testis using MED8 (GB111804) at dilution of 1: 600 |
| Immunofluorescent analysis of paraffin embedded mouse brain using MED8 (GB111804) at dilution of 1: 600 |
| Immunofluorescent analysis of paraffin embedded rat testis using MED8 (GB111804) at dilution of 1: 600 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
