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| Reactivity: | M,R |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | MGMT rabbit polyclonal |
Gene Name: | Methylated-DNA--protein-cysteine methyltransferase |
Synonyms: | MGMT, Mgmt, AGT, AI267024, Agat, O-6-methylguanine-DNA methyltransferase |
Immunogen: | Recombinant protein corresponding to Mouse MGMT |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
IHC | Mouse, Rat | 1: 100-1: 500 | skin, uters, spleen, heart, lymph node |
IF | Mouse, Rat | 1: 100-1: 500 | skin, uters, spleen, heart, lymph node |
Background
O6-alkylguanine DNA alkyltransferase (also known as AGT, MGMT or AGAT) is a protein that in humans is encoded by the O6-methylguanine DNA methyltransferase (MGMT) gene. O6-methylguanine DNA methyltransferase is crucial for genome stability. It repairs the naturally occurring mutagenic DNA lesion O6-methylguanine back to guanine and prevents mismatch and errors during DNA replication and transcription. Accordingly, loss of MGMT increases the carcinogenic risk in mice after exposure to alkylating agents.
Images
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Immunohistochemistry analysis of paraffin-embedded mouse skin using MGMT (GB11380) at dilution of 1:300 |
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Immunohistochemistry analysis of paraffin-embedded mouse uters using MGMT (GB11380) at dilution of 1:300 |
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Immunohistochemistry analysis of paraffin-embedded rat lymph node using MGMT (GB11380) at dilution of 1:300 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |