Your shopping cart is empty!
Anti-MRPL12 Rabbit pAb (100 μl)
| Reactivity: | M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | 39S ribosomal protein L12, mitochondrial |
Synonyms: | L12mt, MRP-L12, 5c5-2, Mitochondrial large ribosomal subunit protein bL12m, MRPL12, MRPL7, RPML12 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human MRPL12 |
Uniprot ID: | Q9DB15 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Mitochondrion |
Predicted MW. / Observed MW. | 21 kDa / 21 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 500-1: 1000 | stomach, colon, breast |
ICC/IF | Human, Mouse | 1: 100-1: 400 | HeLa |
Background
MRPL12 is a component of the 39S subunit of mammalian mitochondrial ribosomes, and is encoded by a nuclear gene. The protein forms a homodimer, localizes predominantly to mitochondria and binds to the large mitochondrial ribosomal subunit. It is required for CycD/Cdk4- induced cell growth. In addition, forming a ribosomal stalk with MRPL7, MRPL12 is required for the recruitment of translation elongation factors Tu and G to the large ribosomal subunit . It also a part of a novel regulatory mechanism that coordinates mitochondrial transcription with translation and/or ribosome biogenesis during human mitochondrial gene expression.
Images
| Western blot analysis of MRPL12 (GB111410) at dilution of 1: 1000 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed HeLa cells using MRPL12(GB111410) at dilution of 1:100-1:400 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed HeLa cells using MRPL12(GB111410) at dilution of 1:100-1:400 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH-3T3 cells using MRPL12(GB111410) at dilution of 1:100-1:400 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
