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Anti-MRPS35 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & M,R & H,M |
| Applications: | WB & IHC/IF & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | 28S ribosomal protein S35 |
Synonyms: | MRP-S35, S35mt, mitochondrial, 28S ribosomal protein S28, DKFZp762P093, MGC104278, OTTHUMP00000240420, MDS023, HDCMD11P |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse MRPS35 |
Uniprot ID: | Q8BJZ4 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Mitochondrion |
Predicted MW. / Observed MW. | 36 kDa / 36 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | kidney, pancreas, heart |
IHC/IF | Mouse, Rat | 1: 400-1: 1200 | kidney |
ICC/IF | Human, Mouse | 1: 50-1: 200 | HeLa |
Background
MRPS35 (mitochondrial ribosomal protein S35), also known as MDS023, MRPS28 or HDCMD11P, is a 323 amino acid protein that localizes to the mitochondrion, where it exists as a component of the 28S ribosomal subunit and works in conjunction with other MRPs to mediate protein synthesis. Existing as two alternatively spliced isoforms, MRPS35 is encoded by a gene located on human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome. This antibody is a rabbit polyclonal antibody raised against the full length MRPS35 protein. It specifically reacts with the 37kd MRPS35protein.
Images
| Western blot analysis of MRPS35 (GB111671) at dilution of 1: 500 |
| Immunohistochemistry of paraffin embedded mouse kidney using MRPS35 (GB111671) at dilution of 1: 400 (300x lens) |
 | Immunohistochemistry of paraffin embedded rat kidney using MRPS35 (GB111671) at dilution of 1: 400 (300x lens) |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed HeLa cells using MRPS35(GB111671) at dilution of 1:50-1:200 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH-3T3 cells using MRPS35(GB111671) at dilution of 1:50-1:200 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
