MTT Cell Proliferation Assay Kit (1000 rxns)

MTT Cell Proliferation Assay Kit provides a standard cell proliferation analysis based on cellular metabolic activity. MTT is an widely established reagent that is used to determine cell number in a variety of applications. Live cells reduce MTT to a strongly pigmented formazan product. After solubilization, the absorbance of the formazan can be measured with a microplate absorbance reader. This kit provides a complete package for performing a cell-counting assay that can detect from 2000 to 250,000 cells, depending on the cell type and conditions. The kit provides a sufficient amount of material for ~1000 assays.
 

Specifications

1. Platform: Microplate Reader

2. Detection Method: Colorimetric

3. OD: 570 nm

Components

1. MTT Reagent: 10× 1 ml
2. Detergent Reagent: 2× 50 ml
 

Storage

Store at 4°C and protect from light.
 

Case Study

Quantitation of Jurkat cells using the MTT Cell Proliferation Assay Kit. Jurkat cells were diluted to the indicated cell numbers in 100 µl volumes, delivered to the wells of a microplate, and incubated for 4 hr with MTT Cell Proliferation Assay Kit, absorbance was measured at 570 nm.