Virus Packaging Services

Virus Packaging Services

With advanced gene synthesis and research platforms, LifeSct independently developed lentivirus (LV), adenovirus (ADV) and adeno-associated virus (AAV) packaging systems. From gene synthesis to virus packaging, we provide a one-stop service, providing high-quality viral vectors suitable for cell infection or in vivo animal experiments.
 

Adenovirus Packaging

LifeSct's adeasy adenovirus packaging system (Ad5 type) offers distinct advantages compared to the admax system. Notably, it facilitates easier detection of false positives of linearized plasmid recombination in the pronucleus. Additionally, the linearized adenovirus plasmid can be utilized in cell lines expressing E1, such as HEK-293 and HEK-293A. Our adenovirus vectors meet the rigorous requirements for both cell infection and in vivo experiments, ensuring reliable results for your research endeavors.

Features:
1. Unsurpassable transduction efficiency (nearly 100% vs. AAV 30~40% and Lentivirus ~30%) in most mammalian cells.
2. No integration into host genome.
3. Compatible with toxic gene of interest (GOI).
4. Induce high-level transient protein expression.
5. Accommodate inserts of up to 7.5 kb.

Required material: A NCBI accession # or gene symbol or plasmid DNA carrying your gene of interest (GOI).

Scale Package Turnaround time Price


 

 

 

Medium

Customized Over-expression Adenovirus (Gene ≤ 3kb),
Deliverables: ≥ 2x1010 PFU/ml, 1 ml
55 business days Quote
RNAi/sgRNA Adenovirus-single target(sgRNA only , no Cas9 gene),
Deliverables: ≥ 2x1010 PFU/ml, 1 ml
45 business days Quote
RNAi/sgRNA Adenovirus-three targets(sgRNA only, no Cas9 gene),
Deliverables: ≥ 2x1010 PFU/ml, 1 ml
45 business days Quote
sgRNA-Cas9 Adenovirus(sgRNA+Cas9),
Deliverables: ≥ 2x1010 PFU/ml, 1 ml
55 business days Quote
miRNA-up/down-Adenovirus,
Deliverables: ≥ 2x1010 PFU/ml, 1 ml
45 business days Quote


 

Large

Customized Over-expression Adenovirus (Gene ≤ 3kb),
Deliverables: ≥ 1011 PFU/ml, 1 ml
60 business days Quote
RNAi/sgRNA Adenovirus-single target(sgRNA only , no Cas9 gene),
Deliverables: ≥ 1011 PFU/ml, 1 ml
50 business days Quote
sgRNA-Cas9 Adenovirus(sgRNA+Cas9),
Deliverables: ≥ 1011 PFU/ml, 1 ml
60 business days Quote


AAV Packaging

Experience LifeSct's superior AAV packaging service. Using plasmid recombination systems and advanced density gradient centrifugation, we perfectly concentrate and purify the virus. Our strict quality control ensures titers up to 10^13 vg/ml, meeting the needs of cell infection and in vivo experiments.

Features:
1. Nonpathogenic with least immune response.
2. Excellent gene delivery efficiency in most cell types including dividing and non-dividing or primary cells.
3. Persistent transgene expression.
4. Multiple serotypes (AAV-1, AAV-2, AAV-3, AAV-4, AAV-5, AAV-6, AAV-7, AAV-8, AAV-9, AAV-DJ/8 and AAV-DJ).

Required material: AAV cis plasmid carrying your gene of interest (GOI), >100 µg.

Scale Package Turnaround time Price


 

Medium

Customized Over-expression AAV (gene ≤ 2.8 kb),
Deliverables: ≥ 1012 vg/ml, 1 ml
25 business days Quote
RNAi/sgRNA AAV(sgRNA only, no Cas9 gene),
Deliverables: ≥ 1012 vg/ml, 1 ml
25 business days Quote
miRNA-up/down-AAV,
Deliverables: ≥ 1012 vg/ml, 1 ml
25 business days Quote



Large

Customized Over-expression AAV (Gene ≤ 2.8kb),
Deliverables: ≥ 1013 vg/ml, 1 ml
25 business days Quote
RNAi/sgRNA AAV (sgRNA only , no Cas9 gene),
Deliverables: ≥ 1013 vg/ml, 1 ml
25 business days Quote


Lentivirus Packaging

Features:
1. Derived from 3rd generation packaging system with a shRNA included for maximum yield.
2. An inhibitory trans element upstream of 5'-LTR allows packaging toxic transgenes.
3. LentiMAX™ packaging cell line is optimized for high pseudoviral titers.
4. Compatible with both 2nd and 3rd generation lentivectors.

Required material: Lentivector (>50 µg) carrying your gene of interests (GOI) in transfection grade.

Scale Package Turnaround time Price


 

 

 

Medium

Customized Over-expression Lentivirus (gene ≤ 3kb),
Deliverables: ≥ 2x108 TU/ml, 200 μl
25 business days Quote
Customized RNAi Lentivirus (single target),
Deliverables: ≥ 2x108 TU/ml, 200 μl
25 business days Quote
Customized RNAi Lentivirus (Design 3 targets, 1 target with guaranteed effect),
Deliverables: ≥ 2x108 TU/ml, 200 μl
25 business days Quote
Customized sgRNA Lentivirus (single target),
Deliverables: ≥ 2x108 TU/ml, 200 μl
25 business days Quote
Customized sgRNA Lentivirus(three targets),
Deliverables: ≥ 2x108 TU/ml, 200 μl
25 business days Quote


 

Large

Customized Over-expression Lentivirus (gene ≤ 3kb),
Deliverables: ≥ 109 TU/ml, 200 μl
30 business days Quote
Customized RNAi Lentivirus,
Deliverables: ≥ 109 TU/ml, 200 μl
30 business days Quote
Customized sgRNA Lentivirus (single target),
Deliverables: ≥ 109 TU/ml, 200 μl
30 business days Quote


Online Quote

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