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Key Features: | • Bright signals with low background • Wide variety of fluorophores • Great for many applications, like Fluorescence in situ Hybridization, TUNEL and more |
Description: | 5-Aminoallyl-UTP (5-[3-aminoallyl]-2'-uridine-5'-triphosphate) is supplied as a 10 mM solution in TE buffer. 5-Aminoallyl-UTP can be enzymatically incorporated into RNA with T7, T3 and SP6 RNA polymerases. The resulting amine-containing RNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin, or hapten. This two-step method for labeling nucleic acids is considerably more economical than the one-step method using a prelabeled UTP. |
Specifications: | 1. Molecular Formula: C12H17N3Na3O15P3 2. Molecular Weight: 605.17 |
Applications: | Enzymatic indirect non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random-primed labeling, or primer extension. |
Component: | 1. 5-Aminoallyl-UTP: 100 μl, 10 mM in TE buffer |
Storage: | At -20°C |
Documents: | Manual |