5-Aminoallyl-dUTP (100 μl)

5-Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as a 10 mM solution in TE buffer. 5-Aminoallyl-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA polymerase, phi29 DNA Polymerase, Klenow Fragment, Klenow Fragment, exo- and DNA Polymerase I. The resulting amine-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin or hapten. This two-step method for labeling nucleic acids is considerably more economical than the one-step method using a prelabeled dUTP.
 

Key Features

• Bright signals with low background.

• Wide variety of fluorophores.

• Great for many applications, like Fluorescence in situ Hybridization, TUNEL and more.
 

Applications

Enzymatic indirect non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random-primed labeling, or primer extension.
 

Specifications

1. Molecular Formula: C12H17N3Na3O14P3
2. Molecular Weight: 589.17
 

Component

1. 5-Aminoallyl-dUTP: 100 μl, 10 mM in TE buffer.
 

Storage

Store at -20°C