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| Key Features: | • Convenience and safety: probe preparation generates no radioactive waste • Stability: probes remain active longer than isotopically labeled probes • Versatility: biotinylated DNA can be used for immobilization and capture procedures via streptavidin binding |
| Description: | 5-FAM-X-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA Polymerase, phi29 DNA Polymerase, Klenow Fragment, exo-, Klenow Fragment, and DNA Polymerase I. The X linker between FAM and dUTP can improve the incorporation efficiency. |
| Specifications: | 1. Ex/Em: 495/518 nm 2. Molecular Formula: C39H38N4Na3O21P3 3. Molecular Weight: 1060.62 |
| Applications: | Enzymatic non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random primed labeling, or primer extension. |
| Component: | 1. 5-FAM-X-dUTP: 25 μl, 1 mM in TE buffer |
| Storage: | At -20°C |
| Documents: | Manual |