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Key Features: | • Convenience and safety: probe preparation generates no radioactive waste • Stability: probes remain active longer than isotopically labeled probes • Versatility: biotinylated DNA can be used for immobilization and capture procedures via streptavidin binding |
Description: | LiFluor 594-X-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA Polymerase, phi29 DNA Polymerase, Klenow Fragment, exo-, Klenow Fragment, and DNA Polymerase I. The X linker between LiFluor 594 and dUTP can improve the incorporation efficiency. |
Specifications: | 1. Ex/Em: 590/615 nm |
Applications: | Enzymatic non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random primed labeling, or primer extension. |
Component: | 1. LiFluor 594-X-dUTP: 25 μl, 1 mM in TE buffer |
Storage: | At -20°C |
Download: | Manual |