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| Key Features: | • Rapid: DNA purification process in less than 20 min post-lysis. • Safe: No organic extraction, no ethanol precipitation. • High-quality: DNA is suitable for a variety of downstream applications. |
| Description: | LiPureTM FFPE DNA Purification Kit is designed for fast and easy purification of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Paraffin removal can be performed using a xylene and ethanol method. Samples are incubated in a specialized lysis buffer along with Proteinase K to reverse crosslinking, effectively releasing short and long DNA fragments. After adjusting the binding conditions with ethanol, the lysate is applied to the DNA Mini column to bind DNA. Cellular debris and proteins are effectively removed during the wash steps. High quality purified DNA is then eluted in elution buffer and is ready for applications such as PCR and next-generation sequencing. |
| Applications: | Genomic DNA isolation from formalin-fixed, paraffin-embedded (FFPE) tissue sections. |
| Technical Specifications: | • Sample Input: Up to 25 mg • Elution Volume: 50-100 μl • Technology: Silica spin column |
| Sample Source: | FFPE tissue |
| Component: | 1. Buffer ATL: 15 ml 2. Buffer DL: 15 ml 3. Buffer AW1: 15 ml 4. Buffer AW2: 15 ml 5. Buffer AE: 15 ml 6. Proteinase K: 1 ml, 20 mg/ml 7. Mini Column: 50 8. 2 ml Collection Tube: 50 |
| Storage: | Buffer & tube at RT; Proteinase K at -20°C |
| Documents: | Manual |
Case Study
Figure 1. Genomic DNA from FFPE samples was isolated using LiPureTM FFPE DNA Purification Kit. Eluted DNA was analyzed on a 1% agarose gel.
Table 1. Genomic DNA yield and quality using LiPureTM FFPE DNA Purification Kit
