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| Key Features: |
• Rapid: DNA purification process in less than 20 min post-lysis. • Safe: No organic extraction, no ethanol precipitation. • High-quality: DNA is suitable for a variety of downstream applications. |
| Description: |
LiPureTM Magbead FFPE DNA Purification Kit is designed for rapid and reliable isolation of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Paraffin removal can be performed using a xylene and ethanol method. Samples are incubated in a specialized lysis buffer along with Proteinase K to reverse crosslinking, effectively releasing short and long DNA fragments. The released DNA is then purified using magnetic bead technology. Purification requires no phenol or chloroform extraction or alcohol precipitation, and involves minimal handling. Purified DNA is suitable for downstream applications including SNP analysis, sequencing, and genotyping. |
| Applications: |
Genomic DNA isolation from formalin-fixed, paraffin-embedded (FFPE) tissue sections. |
| Technical Specifications: |
• Sample Input: Up to 25 mg • Elution Volume: 50-100 μl • Technology: Silica spin column |
| Sample Source: |
FFPE tissue |
| Component: |
1. Buffer ATL: 15 ml 2. Buffer DL: 15 ml 3. Buffer AW1: 15 ml 4. Buffer AW2: 15 ml 5. Buffer AE: 15 ml 6. Proteinase K: 1 ml, 20 mg/ml 7. MagBinding Beads: 2.5 ml |
| Storage: |
Buffer & tube at RT; Proteinase K at -20°C; MagBinding Beads at 2-8°C |
| Documents: |
Manual |
Case Study
Table 1. Genomic DNA yield and quality using LiPureTM Magbead FFPE DNA Purification Kit
