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| Key Features: | • Higher sensitivity: 100 times more sensitive than NanoDrop. • Broad dynamic range: over four orders of magnitude. • More accuracy: unaffected by the presence of proteins, free nucleotides. • Easy to use: mix-and-read format. • Instrument compatibility: assay can be performed using a fluorescence plate reader, or a fluorometer such as Qubit®. |
| Description: | LiQuant™ RNA HS Assay Kit is designed specifically for an easy and accurate quantitation of RNA. The kit is highly selective for RNA in the present of DNA, and offers advantages in stability, linear dynamic range, and sensitivity over other traditional of RNA quantitation. The kit contains concentrated quantitation reagent, dilution buffer, and pre-diluted RNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the fluorescence using fluorescence plate reader or Fluorometer (Figure 1). The assay is well tolerated to common contaminants such as proteins, salts, solvents, detergents, or free nucleotides. The assay can be adapted for use in microplates, tubes or cuvettes. |
| Specifications: | 1. Sample Type: RNA 2. Platform: Fluorescence plate reader or fluorometer 3. Assay: RNA Quantitation, high sensitivity 4. Linear Range: 5-100 ng RNA |
| Applications: | RNA quantitation of samples for microarray, RT-PCR, and Northern blot procedures |
| Component: | 1. LiQuant™ HS RNA Reagent : 1 ml 2. LiQuant™ HS RNA Reagent : 200 ml 3. dsDNA Standard #1: 1 ml 4. dsDNA Standard #2: 1 ml |
| Storage: | At 2-8°C and protect from light |
| Download: | Manual |
Left: Quantitation of ribosomal RNA with LiQuant™ HS RNA Assay Kit using fluorescence plate reader. Right: Quantitation of ribosomal RNA with LiQuant™ HS RNA Assay Kit using Qubit® Fluorometer.