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LiScript™ One Step Green RT-qPCR Kit is meticulously engineered for qPCR detection utilizing RNA templates, such as those from RNA viruses. This kit streamlines the process by enabling both reverse transcription and qPCR within a single tube when Gene Specific Primers (GSP) are present. The elimination of additional opening or pipetting steps significantly enhances assay efficiency and minimizes the risk of contamination.
By integrating the exceptional performance of LiScript™ Reverse Transcriptase and LiTaq™ plus DNA Polymerase with a meticulously optimized buffer, this kit boasts the capability to detect RNA templates as low as 0.1 pg. The buffer's specificity-enhancing factor effectively mitigates primer dimer formation and bolsters the specificity of the product. Conveniently, the kit is supplied as a ready-to-use master mix, simplifying the application process.
Key Features
• Excellent specificity: Include a special additive to inhibit primer dimer formation for higher accuracy.
• High detection sensitivity: High amplification efficiency, allowing detection from 1 pg of total RNA template.
Applications
• PCR
• qPCR
• PCR Cloning
Components
1. RNase free ddH2O: 2× 1.25 mll
2. 2× One Step Green Mix: 2× 1.25 ml
3. One Step Green Enzyme Mix: 250 μl
4. 50× ROX Reference Dye 1: 100 μl
5. 50× ROX Reference Dye 2: 100 μl
Storage
Store at -30 ~ -15°C and transport at ≤ 0°C. For 2× One Step Green Mix, store at -30 ~ -15°C and protect from light.
