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LiTaq™ Super-Fidelity PCR Master Mix is a master mix of LiTaq™ Ultra SuFi DNA Polymerase, dNTP and optimized buffer system at 2× concentration. Therefore, amplification can be carried out as long as primers and templates are added, resulting in less frequent liquid relief operation and higher repeat ability of flux and results. The protective agent in the system guarantees that LiTaq™ Super-Fidelity PCR Master Mix can keep its activity even after many freezing and thawing cycles.
Key Features
• Versatile: Used for routine PCR, or with long, difficult templates.
• Extreme Fidelity: > 100× lower error rate than Taq DNA Polymerase.
• The extension rate is up to 10 sec/kb.
• Robust: Maximum success with minimal optimization, amplifies up to 20kb.
• High Yield: Increased product yield using minimal enzyme.
• No need to change buffer for GC-rich templates.
• More specific and higher yield than most polymerases on market.
Applications
• High fidelity PCR
• High yield and fast PCR
• Blunt end cloning
• Complex templates
• GC-rich PCR
• Site-directed mutagenesis
Component
1. LiTaq™ Super-Fidelity PCR Master Mix: 1 ml
Storage
Store at -20°C.
Case Study
Samples: Human Genomic DNA & cDNA, Blood Samples, Mouse Tail Lysate
LifeSct: LiTaq™ SuFi PCR Master Mix
Competitors: Takara PrimeStar Max, Thermo Platinum Pfx
| PCR reaction setup for amplification: |
PCR cycling for amplification: |
From the above results, it can be concluded that LiTaq™ SuFi is suitable for the amplification of a variety of difficult templates. Under high GC, low template concentration and high inhibitor conditions, LiTaq™ DNA polymerase invariantly performed better than other polymerases. Although direct amplification of lysates is common practice for shorter fragments, amplification of longer fragments is more consistent with LiTaq™ DNA polymerase.
