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Bacterial Protein Expression
The prokaryotic expression system generally takes E. coli as the host cell. Since the cell has advantages like rapid proliferation, high expression, easy purification, good stability, strong anti-pollution ability and low cost, prokaryotic protein expression system is not only the most mature and reliable, but also the most commonly used, the most economical and effective expression system in the experiment of protein expression at present.
With the help of industry-leading codon optimization software and more than ten years' experience in professional protein expression and purification, We provides professional technical support for you in every link of the prokaryotic expression. We not only ensure the expression quantity of your protein, but also ensure the high purity of the delivery according to your needs so that you can focus on the follow-up experiments.
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Specifications
| Process | Description | Delivery Form | Turnaround Time |
| 1. Express vector construction | Express vector construction including codon optimization, gene synthesis and sub cloning. | 1 tube of lyophilized plasmid (4ug/tube), 1 tube of puncture strains | 10-20 business days |
| 2. Optimization: expression conditions | Under the premise of ensuring sequence correction, the influence of host bacteria on the expression effect is also very obvious. We will transform recombinant plasmids with accurate sequences into the optimal expression host bacteria, including BL21 (DE3), Arctic Express (DE3), TOP10F, Rosetta (DE3) pLySs, Rosetta-gami B (DE3) and those strains with special skills. We will select at least 6 positive colonies to conduct expression laboratory scale test and optimization after transformation. During the test process, we will optimize the culture conditions which are suitable for various strains, including temperature, culture medium, inducer type, induction time, induction quantity and other factors. After the testing of expression quantity and solubility through SDS-PAGE, we offer feasibility suggestions about whether or not to enlarge and the enlarge scale. | A report of laboratory scale test verification | 5 business days |
| 3. Protein expression and purification | To optimize and obtain the genes with appropriate expression condition and use 1L or greater culture scale to induce and express recombinant bacteria. After the culture and induction, affinity purification or ion exchange, hydrophobic gel and multi-step purification (optional or alternative) are further conducted. It is tacitly approved that SDS-PAGE tests purity and Western blot tests accuracy (optional, the customer provides verified first antibody). | 1-10 mg recombinant proteins and purification report data | 10-15 business days |
| 4. Protein post processing (optional) | For those proteins with further special requirements, we provides you a variety of post processing options, including protein tag removal after purification, filtration sterilization, endotoxin removal, freeze-dry, N-terminal sequencing and other services. | Inquiry | Inquiry |
