PCR Cloning & Subcloning

The LiClone™ platform revolutionizes DNA sequence amplification through its dual-mode architecture supporting both PCR cloning and precision subcloning workflows. Our patented directional cloning technology enables seamless cloning into any vector system (plasmid, viral, or custom backbones).
 

Key Features

• Quickly produce your desired gene constructs with 100% accuracy, verified by Sanger sequencing.

• Guarantee successful clone at any site in any vector system of interest with special amplified primer design.

• Professional technical support offers you proactive communications on your project processing status.
 

Online Quote

Please click the button on the right side and login to your account to request a quote.
 

Service Options (Cat. #: S0022)

* Turnaround time is dependent on length and complexity of the gene fragments. In a few cases, shipping time may exceed the estimated time.
** For plasmid >10 kb, need to quote
 

Sample Requirements

1. DNA sequence: If the sequence has not been verified, we would do sequence validation as required (Additional fees will be charged).

2. Vector: Commercial vectors are preferred. For specific vectors (contrast to commercial ones) constructed by the customer, full length sequence is needed. If there is no sequence information, we would do sequence validation as required (Additional fees will be charged).

3. If rare restriction endonucleases or special molecular biological reagents are used in the experiment, customers may be requested to provide these reagents. we can purchase them for you, and the additional cost for these reagents will be charged. All purchased reagents will be returned to customers after the delivery.
 

Delivery Forms

1. Lyophilized plasmid DNA (2-5 µg)
2. Sequencing chromatogram
3. Certificate of analysis (COA)