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Anti-Myelin Basic Protein Mouse mAb (100 μl)
| Reactivity: | H,M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Myelin Basic Protein |
Synonyms: | MBP, entrez:4155, myelin basic protein |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Myelin Basic Protein |
Uniprot ID: | P04370 |
Isotype: | IgG1,κ |
Purity: | Affinity purification |
Subcellular location: | Myelin membrane, Cytoplasmic side of myelin |
Predicted MW. / Observed MW. | 27 kDa / 17/20 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 2000-1: 4000 | brain, cerebral cortex, cerebellum |
IHC/IF | Mouse, Rat | 1: 3000-1: 6000 | brain, hippocampus |
Background
The classic group of MBP isoforms (isoform 4-isoform 13) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells.
Images
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Western blot analysis of Myelin Basic Protein (GB12226) at dilution of 1: 2000 |
| Immunohistochemistry of paraffin embedded mouse brain using MBP (GB12226) at dilution of 1: 3000 (400x lens) |
| Immunohistochemistry of paraffin embedded rat brain using MBP (GB12226) at dilution of 1: 3000 (400x lens) |
 | Immunofluorescence of paraffin embedded rat hippocampus using Neun (GB11138) (green) + Iba1 (GB12105) (red) + MBP (GB12226) (spred) + GFAP (GB11096) (yellow) at dilution of 1: 1500 (400x lens) |
 | Immunofluorescence of paraffin embedded mouse brain using MBP (GB12226) at dilution of 1: 1500 (400x lens) |
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Immunofluorescence of paraffin embedded rat brain using MBP (GB12226) at dilution of 1: 1500 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
