Reactivity: | H,M,R & M,R |
Applications: | WB & IHC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | NCBP2 rabbit polyclonal antibody |
Gene Name: | Nuclear cap-binding protein subunit 2 |
Synonyms: | 20 kDa nuclear cap-binding protein, NCBP 20 kDa subunit, CBP20, Ncbp2, Cbp20, PIG55 |
Immunogen: | Recombinant protein corresponding to Mouse NCBP2 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Nucleus |
Predicted MW. | 18 kDa |
Observed MW. | 16 kDa |
Uniprot ID: | P52298, Q9CQ49, B1WC40 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 300-1: 600 | testis, colon, brain |
IHC/IF | Mouse, Rat | 1: 650-1: 1300 | corpus striatum, prefrontal lobe |
Background
The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. Multiple transcript variants encoding different isoforms have been found for this gene.
Images
Western blot analysis of NCBP2 (GB112277) at dilution of 1: 300 | |
Immunofluorescence of paraffin embedded mouse prefrontal lobe using NCBP2 (GB112277) at dilution of 1: 650 (400x lens) | |
Immunofluorescence of paraffin embedded rat corpus striatum using NCBP2 (GB112277) at dilution of 1: 650 (400x lens) |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |