Your shopping cart is empty!
-23%
Anti-NF-kB p65 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Transcription factor p65 |
Synonyms: | RELA, NFKB3, p65, RELA proto-oncogene, NF-kB subunit |
Immunogen: | Recombinant protein corresponding to Mouse NF-κB p65 |
Uniprot ID: | Q04207 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Nucleus |
Predicted MW. / Observed MW. | 65 kDa / 70 kDa |
Applications
WB | Human, Mouse, Rat | 1: 500-1: 1000 | lung, thymus |
ICC/IF | Human, Mouse, Rat | 1: 1500-1: 3000 | HeLa, NIH/3T3, PC-12 |
Background
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one.
Images
| Western blot analysis of NF-kB p65 (GB11997) at dilution of 1: 500 |
| Western blot analysis of NF-kB p65 (GB11997) at dilution of 1: 500 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed HeLa cells using NF-κB p65 (GB11997) at dilution of 1: 3000 Left: HeLa cells untreated Right: HeLa cells were treated with 20ng/mL TNFα for 20min |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH/3T3 cells using NF-κB p65 (GB11997) at dilution of 1: 3000 Left: NIH/3T3 cells untreated Right: NIH/3T3 cells were treated with 20ng/mL TNFα for 20min |
 | Immunocytochemistry analysis of 4% paraformaldehyde-fixed PC-12 cells using NF-κB p65 (GB11997) at dilution of 1: 3000 Left: PC-12 cells untreated Right: PC-12 cells were treated with 20ng/mL TNFα for 20min |
Storage
| Storage | Store at -20 °C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
