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Anti-Neurofibromin Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Neurofibromin rabbit polyclonal antibody |
Gene Name: | Neurofibromin |
Synonyms: | Neurofibromatosis-related protein NF-1, Nf1, Neurofibromin, neurofibromin 1, NFNS, VRNF, WSS |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Neurofibromin |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. | 320 kDa |
Observed MW. | 320 kDa |
Uniprot ID: | P21359, Q04690, P97526 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | lung |
IHC/IF | Human, Mouse, Rat | 1: 750-1: 4000 | brain, breast cancer, colon, lung, ovary, Skeletal muscle, testis |
Background
Neurofibromin 1 (NF1), the Nf1 gene product, is a large protein which contains a central Ras-GTPase-activating (RasGAP) domain and is suggested to function as a negative regulator of proto-oncogene RAS and downstream effectors. It stimulates the GTPase activity of RAS and acts as regulator of RAS signaling. Consistent with its biochemical activity, NF1 functions as a tumor-suppressor gene in juvenile myelomonocytic leukemia (JMML), and diseased BM cells exhibit aberrant Raf/MEK/ERK signaling. Moreover, NF1 shows greater affinity for Ras GAP, but lower specific activity.
Images
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Western blot analysis of Neurofibrmin-1 (GB111112) at dilution of 1: 500 |
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Immunohistochemistry of paraffin embedded mouse brain using Neurofibinin (GB111112) at dilution of 1: 2000 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse colon using Neurofibinin (GB111112) at dilution of 1: 2000 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse lung using Neurofibinin (GB111112) at dilution of 1: 2000 (400x lens) |
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Immunohistochemistry of paraffin embedded rat colon using Neurofibinin (GB111112) at dilution of 1: 2000 (400x lens) |
 | Immunohistochemistry of paraffin embedded rat lung using Neurofibinin (GB111112) at dilution of 1: 2000 (400x lens) |
 | Immunohistochemistry of paraffin embedded rat skeletal muscle using Neurofibinin (GB111112) at dilution of 1: 2000 (400x lens) |
 | Immunofluorescence of paraffin embedded human breast cancer using Neurofibinin (GB111112) at dilution of 1: 1500 (400x lens) |
 | Immunofluorescence of paraffin embedded mouse brain using Neurofibinin (GB111112) at dilution of 1: 1500 (400x lens) |
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Immunofluorescence of paraffin embedded mouse ovary using Neurofibinin (GB111112) at dilution of 1: 1500 (400x lens) |
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Immunofluorescence of paraffin embedded rat testis using Neurofibinin (GB111112) at dilution of 1: 1500 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
