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Anti-PARP1 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | PARP1 rabbit polyclonal antibody |
Gene Name: | Poly [ADP-ribose] polymerase 1 |
Synonyms: | PARP 1, PARP-1, PARP1, ADPRT, ADPRT1, msPARP, PPOL, ARTD1, PARP, PARP-1, pADPRT-1, poly (ADP ribose) polymerase 1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human PARP1 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. | 113 kDa |
Observed MW. | 116 kDa |
Uniprot ID: | P09874, P11103, P27008 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | HEK293, Jurkat, MCF-7, HeLa, HeLa treated by staurosporine, NIH/3T3, HepG2 |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 2500 | brain, colon cancer, epityphlon, heart, kidney, placenta, testis |
Background
PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis.
Images
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Western blot analysis of PARP (GB111501) at dilution of 1: 500 |
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Immunohistochemistry of paraffin embedded human colon cancer using PARP (GB111501) at dilution of 1: 1200 (400x lens) |
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Immunohistochemistry of paraffin embedded human epityphlon using PARP (GB111501) at dilution of 1: 1200 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse brain using PARP (GB111501) at dilution of 1: 1200 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse placenta using PARP (GB111501) at dilution of 1: 1200 (400x lens) |
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Immunohistochemistry of paraffin embedded rat brain using PARP (GB111501) at dilution of 1: 1200 (400x lens) |
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Immunohistochemistry of paraffin embedded rat kidney using PARP (GB111501) at dilution of 1: 1200 (400x lens) |
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Immunohistochemistry of paraffin embedded rat testis using PARP (GB111501) at dilution of 1: 1200 (400x lens) |
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Immunofluorescence of paraffin embedded rat heart using PARP (GB111501) at dilution of 1: 1200 (400x lens) |
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Immunofluorescence of paraffin embedded rat placenta using PARP (GB111501) at dilution of 1: 1200 (400x lens) |
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Immunofluorescence of paraffin embedded rat testis using PARP (GB111501) at dilution of 1: 1200 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
