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Anti-PCNA Mouse mAb (100 μl)
| Reactivity: | H,M,R & H,M,R & H,M,R |
| Applications: | WB & IHC/IF & ICC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Proliferating cell nuclear antigen |
Synonyms: | PCNA, ATLD2, proliferating cell nuclear antigen |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse PCNA |
Uniprot ID: | P17918 |
Isotype: | IgG2a,κ |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. / Observed MW. | 29 kDa / 36 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | testis, placenta |
IHC/IF | Human, Mouse, Rat | 1: 1000-1: 4000 | colon, rectum, spleen, gastric cancer |
ICC/IF | Human, Mouse, Rat | 1: 100-1: 500 | HeLa |
Background
Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics.
Images
| WB analysis of PCNA (GB12010). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 3000, RT, 1h. |
| IHC analysis of PCNA (GB12010). Sample: Human gastric cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
| IHC analysis of PCNA (GB12010). Sample: Human rectum (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
 | IHC analysis of PCNA (GB12010). Sample: Mouse colon (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
 | IHC analysis of PCNA (GB12010). Sample: Mouse spleen (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
| IHC analysis of PCNA (GB12010). Sample: Mouse testis (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
| IHC analysis of PCNA (GB12010). Sample: Rat spleen (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
| IHC analysis of PCNA (GB12010). Sample: Rat testis (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
| ICC analysis of PCNA (GB12010). Sample: HeLa, 4% PFA (G1101) 20min. Antigen retrieval: 0.5% Triton X-100 (G1204), RT, 15 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 200, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300 RT, 1h. |
| ICC analysis of PCNA(GB12010)red. Sample: HeLa, 4% PFA (G1101) 20min. Primary antibody: 1: 200, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
