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Anti-PCNA Rabbit pAb (100 μl)
| Reactivity: | H & H,M |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | PCNA rabbit polyclonal antibody |
Gene Name: | Proliferating cell nuclear antigen |
Synonyms: | PCNA, ATLD2, proliferating cell nuclear antigen |
Uniprot ID: | Q12004, P17918, P04961 |
Immunogen: | Recombinant protein corresponding to Mouse PCNA |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. | 29 kDa |
Observed MW. | 36 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human | 1: 500-1: 2000 | testis, colon |
IHC/IF | Human, Mouse | 1: 400-1: 2000 | lung cancer, ovarian cancer, testis |
Background
Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics.
Images
| Western blot analysis of PCNA (GB11010-1) at dilution of 1: 500 |
| Immunofluorescence of paraffin embedded human breast cancer using PCNA (GB11010-1) at dilution of 1: 400 (300x lens) |
| Immunofluorescence of paraffin embedded human breast cancer using PCNA (GB11010-1) at dilution of 1: 400 (400x lens) |
| Immunofluorescence of paraffin embedded mouse testis using PCNA (GB11010-1) at dilution of 1: 400 (250x lens) |
Storage
| Storage | Store at -20 °C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
