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| Reactivity: | Human |
| Applications: | WB, IF/IC |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | tumor protein p53 inducible protein 11 |
| Gene Symbol: | TP53I11 |
| Synonyms: | PIG11 |
| Immunogen: | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PIG11. The exact sequence is proprietary. |
| Dilution: | WB 1:500-1:1000; IF/IC 1:100-1:500 |
| Gene ID: | 9537 |
| UniProt ID: | O14683 |
| Purification Method: | The antibody was purified by immunogen affinity chromatography. |
| Concentration: | 1mg/mL |
| Buffer: | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Storage: | Store at -20°C. Avoid repeated freeze / thaw cycles. |
| Documents: | Manual-PIG11 Polyclonal Antibody |
Images
| Western blot analysis of PIG11 expression in HuvEc (A) whole cell lysates. |
| Immunohistochemistry analysis of tumor protein p53 inducible protein 11 |
| Immunofluorescent analysis of PIG11 staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). |
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