Anti-Placental alkaline phosphatase (PLAP) Mouse mAb (100 μl)

Alkaline phosphatase, placental type

Synonyms:

Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1, ALPP, PLAP

Immunogen:

Human recombinant PLAP protein expressed in HEK293 cells

Uniprot ID:

P05187

Isotype:

lgG

Purity:

Affinity purification

Subcellular location:

Cell membrane

Predicted MW. / Observed MW.

58 kDa / 66-68 kDa

WB

Human

1: 500-1: 1000

HeLa, Hep G2

IHC/IF

Human

1: 500-1: 1500

placenta

WB analysis of PLAP (GB122546).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 3000, RT, 1h.

IHC analysis of PLAP (GB122546).
Sample: Human placenta (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 3000, RT, 1h.