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| Reactivity: | H,M,R |
| Applications: | IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Mismatch repair endonuclease PMS2 |
Synonyms: | PMS2 PMSL2 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human Postmeiotic Segregation Increased 2 |
Uniprot ID: | P54278 |
Isotype: | IgG2b,κ |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Product Usage Information
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | colon, small intestine |
Background
Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2.
Images
| IHC analysis of PMS2(GB120001). Sample: Human colon,4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1:3000, 4°Covernight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200, RT, 1h. |
| IHC analysis of PMS2(GB120001). Sample: Human small intestine,4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min . Primary antibody: 1:3000, 4°Covernight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT, 1h. |
| IHC analysis of PMS2(GB120001). Sample: Mousekidney,4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min . Primary antibody: 1:3000, 4°Covernight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT, 1h. |
| IHC analysis of PMS2(GB120001). Sample: Mousesmal lintestine,4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1:3000, 4°Covernight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT, 1h. |
| IHC analysis of PMS2(GB120001). Sample: Mousetestis,4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min . Primary antibody: 1:3000, 4°Covernight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT, 1h. |
| IHC analysis of PMS2(GB120001). Sample: Ratcolon,4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min . Primary antibody: 1:3000, 4°Covernight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT, 1h. |
| IHC analysis of PMS2(GB120001). Sample: Ratkidney,4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min . Primary antibody: 1:3000, 4°Covernight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT, 1h. |
| IHC analysis of PMS2(GB120001). Sample: Rat testis,4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min . Primary antibody: 1:3000, 4°Covernight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
