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Anti-PRPF4 Rabbit pAb (100 μl)
| Reactivity: | H,M |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | PRPF4 rabbit polyclonal antibody |
Gene Name: | U4/U6 small nuclear ribonucleoprotein Prp4 |
Synonyms: | U4/U6 snRNP 60 kDa protein, WD splicing factor Prp4, Prpf4, Prp4p, HPRP4P |
Immunogen: | Recombinant protein corresponding to Mouse PRPF4 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. | 58 kDa |
Observed MW. | 58 kDa |
Uniprot ID: | O43172, Q9DAW6 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | brain, spleen |
IHC/IF | Human, Mouse, Rat | 1: 400-1: 1000 | bladder, brain, esophagus, gastric cancer, kidney, liver, lung, ovary, spleen |
Background
PRPF4, also named as U4/U6 small nuclear ribonucleoprotein Prp4, is a 522 amino acid protein, which contains seven WD repeats. PRPF4 localizes in the Nucleus speckle. PRPF4 participates in pre-mRNA splicing and is a part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.PRPF4-mediated phosphorylation of KLF13 plays a role in the regulation of CCL5 expression in T lymphocytes.
Images
| Western blot analysis of PRPF4 (GB112280) at dilution of 1: 500 |
| Immunohistochemistry of paraffin embedded human esophagus using PRPF4 (GB112280) at dilution of 1: 450 (400x lens) |
| Immunohistochemistry of paraffin embedded human gastric cancer using PRPF4 (GB112280) at dilution of 1: 450 (400x lens) |
| Immunohistochemistry of paraffin embedded mouse bladder using PRPF4 (GB112280) at dilution of 1: 450 (400x lens) |
| Immunohistochemistry of paraffin embedded mouse brain using PRPF4 (GB112280) at dilution of 1: 450 (400x lens) |
| Immunohistochemistry of paraffin embedded mouse kidney using PRPF4 (GB112280) at dilution of 1: 450 (400x lens) |
| Immunohistochemistry of paraffin embedded mouse lung using PRPF4 (GB112280) at dilution of 1: 450 (400x lens) |
| Immunohistochemistry of paraffin embedded rat bladder using PRPF4 (GB112280) at dilution of 1: 450 (400x lens) |
| Immunohistochemistry of paraffin embedded rat brain using PRPF4 (GB112280) at dilution of 1: 450 (400x lens) |
| Immunohistochemistry of paraffin embedded rat kidney using PRPF4 (GB112280) at dilution of 1: 450 (400x lens) |
| Immunohistochemistry of paraffin embedded rat lung using PRPF4 (GB112280) at dilution of 1: 450 (400x lens) |
| Immunofluorescence of paraffin embedded mouse bladder using PRPF4 (GB112280) at dilution of 1: 500 (400x lens) |
| Immunofluorescence of paraffin embedded mouse brain using PRPF4 (GB112280) at dilution of 1: 500 (400x lens) |
| Immunofluorescence of paraffin embedded mouse lung using PRPF4 (GB112280) at dilution of 1: 500 (400x lens) |
| Immunofluorescence of paraffin embedded mouse ovary using PRPF4 (GB112280) at dilution of 1: 500 (400x lens) |
| Immunofluorescence of paraffin embedded rat kidney using PRPF4 (GB112280) at dilution of 1: 500 (400x lens) |
| Immunofluorescence of paraffin embedded rat liver using PRPF4 (GB112280) at dilution of 1: 500 (400x lens) |
| Immunofluorescence of paraffin embedded rat spleen using PRPF4 (GB112280) at dilution of 1: 500 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
