Your shopping cart is empty!
Anti-Phospho-FAK (Tyr397) Rabbit pAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Focal adhesion kinase 1 |
Synonyms: | PTK2, FADK, FAK, FAK1, FRNK, PPP1R71, p125FAK, pp125FAK, protein tyrosine kinase 2, P-FAK |
Immunogen: | KLH conjugated Synthetic phosphopeptide corresponding to Human FAK（Tyr397) |
Uniprot ID: | Q05397 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 119,114,99,63,49,120 kDa / 125 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 300-1: 600 | HeLa, NIH/3T3, PC-12 |
Background
FAK is a protein of 125 kD recruited as a participant in focal adhesion dynamics between cells, and has a role in motility and cell survival. FAK is a highly conserved, non-receptor tyrosine kinase originally identified as a substrate for the oncogene protein tyrosine kinase v-src. This cytosolic kinase has been implicated in diverse cellular roles including cell locomotion, mitogen response and cell survival.
Images
| WB analysis of Phospho-FAK (Tyr397)（GB115455). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody:1:300, 4°C overnight.. Secondary antibody:HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
