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Anti-Phospho-NF-kB p65 (S536) Rabbit pAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Phospho- NF-kB p65 (S536) rabbit polyclonal antibody |
Gene Name: | Transcription factor p65 |
Synonyms: | NF Kappa B, NF κB, NFKB 3, NFKB p65, NFKB3, NF-κB, NF-κB3, p65, RELA, Transcription factor p65, p-p65 |
Uniprot ID: | Q04206 |
Immunogen: | KLH conjugated Synthetic phosphopeptide corresponding to Human NF-kB p65 (S536) |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 65 kDa |
Observed MW. | 70 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 300-1: 600 | HeLa, MCF7, NIH/3T3, PC12 |
Background
Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators.
Images
| Western blot analysis of phospho-p65 (S536) (GB113882) at dilution of 1: 600 Lane1: HeLa untreated Lane2: HeLa treated with 20ng/mL TNFα for 20min, whole cell lysate Lane3: MCF7 untreated Lane4: MCF7 treated with 20ng/ml TNFα for 20min, whole cell lysate |
| Western blot analysis of phospho-p65 (S536) (GB113882) at dilution of 1: 600 Lane1: HeLa untreated Lane2: HeLa treated with 20ng/mL TNFα for 20min, whole cell lysate Lane3: MCF7 untreated Lane4: MCF7 treated with 20ng/ml TNFα for 20min, whole cell lysate The figure above was treated with phospho-blocking pepide, the figure below was treated with non-phospho- blocking pepide |
| Western blot analysis of Phospho-NF-κBp65 (Ser536) GB113882 at dilution of 1: 300 Lane1: NIH/3T3 untreated Lane2: NIH/3T3 treated with 40ng/mL TNFα for 20min |
| Western blot analysis of Phospho-NF-κBp65 (Ser536) GB113882 at dilution of 1: 300 Lane1: PC12 untreated Lane2: PC12 treated with 40ng/mL TNFα for 20min |
Storage
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
