Lentivirus EF1a-FLPo-Puro (2× 25 µl)

FLP originally identified from Saccharomyces cerevisiae is a site-specific recombinases (SSR) from the λ integrase family and recognizes distinct 34 bp FRT sequence. It has been widely used for generating conditional knockout mice, mediated by FLP/FRT system. Initial use of FLP in mammalian cells revealed inefficient recombinase activity due to thermo-instability of the FLP protein. Subsequent screening for thermo-stable mutants resulted in the identification of FLPe which was 4-fold increase in recombination efficiency at 37 °C. However the recombination efficiency of FLPe in cells remains very low because of its non-mammalian origin.

FLPo is a mouse codon optimized FLPe that greatly increases the FRT recombination efficiency for both in vivo and in vitro in mouse. This LV-EF1α-FLPo-Puro pre-made lentivirus expresses FLPo recombinase with co-expression of puromycin selection in an IRES cassette under EF1α promoter. Ready to use format.