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| Product Category: | Premade recombinant lentivirus; LV-EF1α-SpCas9; LV-EF1α-Cas9; LV-EF1A-SpCas9; LV-EF1A-Cas9; EF1A-Cas9 Lentivirus |
| Description: | Lentivirus EF1α-SpCas9 is a pre-made lentivirus which over-expresses SpCas9 under the EF1α promoter and can be used along with guided RNA for genome-editing. The Type II prokaryotic CRISPR/Cas system is the new class of tools for targeted genome engineering. Cas9 (CRISPR associated protein 9) is an RNA-guided DNA nuclease enzyme associated with Streptococcus pyogenes' CRISPR immunity system. The cas9 nucleases derived from clustered regularly interspaced short palindromic repeats (CRISPR)-cas systems uses small RNAs as guides (gRNA) to cleave DNA in a sequence-specific manner. With its ease in designing guide sequences to target specific genomic loci, the CRISPR/Cas system is a much simpler, faster, and robust alternative to TALEN and Zinc finger nuclease platforms. Ready to use format. |
| Titer: | > 1x109 TU/mL |
| Selection Antibiotic: | None |
| Purity: | In vivo grade and OK for both in vitro tissue culture infection and in vivo injection |
| Storage Buffer: | PBS |
| Documents: | Manual |
