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Anti-160 kDa Neurofilament Medium Mouse mAb (100 μl)
| Reactivity: | M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Neurofilament medium polypeptide |
Synonyms: | 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein, Nef3, Nefm, NF-M, Nfm, 150kDa medium |
Immunogen: | Recombinant protein corresponding to Mouse NF-M |
Uniprot ID: | P08553 |
Isotype: | IgG1,κ |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Cytoskeleton |
Predicted MW. / Observed MW. | 96 kDa / 150 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Mouse, Rat | 1: 500-1: 1000 | brain, cerebral cortex, hippocampus, spinal cord |
IHC/IF | Mouse, Rat | 1: 500-1: 4000 | brain, spinal cord, epencephalon |
Background
NEFM, also named as NEF3 and NFM, belongs to the intermediate filament family. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Images
| Western blot analysis of NF-M (GB12763) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded mouse brain using NF-M (GB12763) at dilution of 1: 4000 |
 | Immunohistochemistry analysis of paraffin-embedded rat brain using NF-M (GB12763) at dilution of 1: 4000 |
 | Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using NF-M (GB12763) at dilution of 1: 4000 |
 | Immunohistochemistry analysis of paraffin-embedded rat spinal cord using NF-M (GB12763) at dilution of 1: 4000 |
 | Immunohistochemistry analysis of paraffin-embedded mouse epencephalon using NF-M (GB12763) at dilution of 1: 4000 |
 | Immunohistochemistry analysis of paraffin-embedded rat epencephalon using NF-M (GB12763) at dilution of 1: 4000 |
 | Immunofluorescent analysis of paraformaldehyde-fixed mouse brain using NF-M (GB12763) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed rat brain using NF-M (GB12763) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed mouse spinal cord using NF-M (GB12763) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed rat spinal cord using NF-M (GB12763) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
