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Anti-ACADM/MCAD Rabbit pAb (100 μl)
| Reactivity: | M,R & H,M & M |
| Applications: | WB & IHC/IF & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Medium-chain specific acyl-CoA dehydrogenase, mitochondrial |
Synonyms: | MCAD, Acadm, ACAD1, Acyl coenzyme A dehydrogenase, MCADH, Medium chain acyl CoA dehydrogenase |
Immunogen: | Recombinant protein corresponding to Mouse ACADM |
Uniprot ID: | P45952 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Mitochondrion |
Predicted MW. / Observed MW. | 46 kDa / 43 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 2000-1: 3000 | heart, liver, kidney |
IHC/IF | Human, Mouse | 1: 500-1: 1500 | liver |
ICC/IF | Mouse | 1: 100-1: 300 | NIH3T3 |
Background
ACADM protein is a medium chain specific (C4 to C12 straight chain) acyl Coenzyme A dehydrogenase. The enzyme catalyzes the initial step of the mitochondrial fatty acid beta oxidation pathway. ACADM expression is induced during periods of fasting, when reliance on fatty acids for energy is increased. Clinical phenotypes are associated with ACADM hereditary deficiency.
Images
| WB analysis of ACADM (GB112107). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 2000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| IF analysis of ACADM (GB112107)red. Sample: Human gastric cancer, 4% PFA (G1101) 12-24h. Blocking buffer: Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 1500, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
| IF analysis of ACADM (GB112107)red. Sample: Mouse liver, 4% PFA (G1101) 12-24h. Blocking buffer: Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 1500, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
| IF analysis of ACADM (GB112107)red. Sample: Mouse kidney, 4% PFA (G1101) 12-24h. Blocking buffer: Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 1500, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
| ICC analysis of ACADM (GB112107)red. Sample: NIH3T3, 4% PFA (G1101) 20min. Blocking buffer: Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 200, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
