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Anti-ACAT1 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | ACAT1 rabbit polyclonal antibody |
Gene Name: | Acetyl-CoA acetyltransferase, mitochondrial |
Synonyms: | Acetoacetyl-CoA thiolase, Acat1, THIL, MAT, ACAT, RATACAL |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse ACAT1 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Mitochondrion |
Predicted MW. | 45 kDa |
Observed MW. | 45 kDa |
Uniprot ID: | P24752, Q8QZT1, P17764 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | kidney, brain, colon |
IHC/IF | Human, Mouse, Rat | 1: 600-1: 2000 | colon, epididymis, heart, kidney, testis |
Background
This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.
Images
| Western blot analysis of ACAT1 (GB112323) at dilution of 1: 1000 |
| Immunohistochemistry of paraffin embedded human colon using ACAT1 (GB112323) at dilution of 1: 650 (400x lens) |
| Immunohistochemistry of paraffin embedded human kidney using ACAT1 (GB112323) at dilution of 1: 650 (400x lens) |
| Immunofluorescence of paraffin embedded human kidney using ACAT1 (GB112323) at dilution of 1: 750 (400x lens) |
| Immunofluorescence of paraffin embedded mouse kidney using ACAT1 (GB112323) at dilution of 1: 650 (400x lens) |
| Immunofluorescence of paraffin embedded mouse testis using ACAT1 (GB112323) at dilution of 1: 650 (300x lens) |
| Immunofluorescence of paraffin embedded rat colon using ACAT1 (GB112323) at dilution of 1: 650 (400x lens) |
 | Immunofluorescence of paraffin embedded rat epididymis using ACAT1 (GB112323) at dilution of 1: 650 (400x lens) |
 | Immunofluorescence of paraffin embedded rat heart using ACAT1 (GB112323) at dilution of 1: 650 (400x lens) |
| Immunofluorescence of paraffin embedded rat kidney using ACAT1 (GB112323) at dilution of 1: 650 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
