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Anti-alpha Actinin 1/ACTN1 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Alpha-actinin-1 |
Synonyms: | Alpha-actinin cytoskeletal isoform, F-actin cross-linking protein, Non-muscle alpha-actinin-1, ACTN1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human alpha Actinin 1/ACTN1 |
Uniprot ID: | P12814 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane, Cytoplasm |
Predicted MW. / Observed MW. | 103 kDa / 103 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 800 | kidney, skeletal muscle, uterine |
IHC/IF | Human, Mouse, Rat | 1: 300-1: 1000 | brain, heart, skeletal muscle |
Background
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane.This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene.
Images
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Western blot analysis of ACTN1 (GB111557) at dilution of 1: 800 |
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Immunohistochemistry analysis of paraffin embedded mouse heart using ACTN1 (GB111557) at dilution of 1: 300 |
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Immunofluorescence analysis of paraffin embedded rat skeletal muscle using ACTN1 (GB111557) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
