Reactivity: | M,R & M,R & M,R |
Applications: | WB & IHC & IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | ADA rabbit polyclonal |
Gene Name: | Adenosine deaminase |
Synonyms: | Adenosine aminohydrolase, ADA |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse ADA |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | |
Predicted MW. | 40 kDa |
Observed MW. | 43 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Mouse, Rat | 1: 750-1: 15000 | stomach, small intestine |
IHC | Mouse, Rat | 1: 1000-1: 2000 | embryo |
IF | Mouse, Rat | 1: 400-1: 800 | spleen, duodenum, stomach, embryo |
Background
This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia.
Images
Western blot analysis of ADA (GB11209) at dilution of 1: 1500 Lane 1: Mouse stomach tissue lysate Lane 2: Mouse small intestine tissue lysate Lane 3: Rat small intestine tissue lysate | |
Immunohistochemistry analysis of paraffin-embedded mouse embryo using ADA (GB112099) at dilution of 1: 2000 | |
Immunohistochemistry analysis of paraffin-embedded rat embryo using ADA (GB112099) at dilution of 1: 2000 | |
Immunofluorescent analysis of paraformaldehyde-fixed mouse spleen using ADA (GB112099) at dilution of 1: 800 | |
Immunofluorescent analysis of paraformaldehyde-fixed rat spleen using ADA (GB112099) at dilution of 1: 800 | |
Immunofluorescent analysis of paraformaldehyde-fixed mouse duodenum using ADA (GB112099) at dilution of 1: 800 | |
Immunofluorescent analysis of paraformaldehyde-fixed mouse stomach using ADA (GB112099) at dilution of 1: 800 | |
Immunofluorescent analysis of paraformaldehyde-fixed rat embryo using ADA (GB112099) at dilution of 1: 800 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |