Your shopping cart is empty!
Anti-AP endonuclease 2 Rabbit pAb (100 μl)
| Reactivity: | H,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | DNA-(apurinic or apyrimidinic site) endonuclease 2 |
Synonyms: | Ape2, APEXL2, APEX2, APEX nuclease 2, AP endonuclease 2, XTH2 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse AP endonuclease 2 |
Uniprot ID: | Q68G58 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 57 kDa / 57 kDa |
Product Usage Information
WB | Human, Rat | 1: 1000-1: 2000 | thymus |
Background
Functions as a weak apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Displays also double-stranded DNA 3'-5' exonuclease, 3'-phosphodiesterase activities. Shows robust 3'-5' exonuclease activity on 3'-recessed heteroduplex DNA and is able to remove mismatched nucleotides preferentially. Shows fairly strong 3'-phosphodiesterase activity involved in the removal of 3'-damaged termini formed in DNA by oxidative agents. In the nucleus functions in the PCNA-dependent BER pathway. Required for somatic hypermutation (SHM) and DNA cleavage step of class switch recombination (CSR) of immunoglobulin genes. Required for proper cell cycle progression during proliferation of peripheral lymphocytes.
Images
| Western blot analysis of APEX2 (GB114854) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
