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| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | ATG4A rabbit polyclonal antibody |
Gene Name: | Cysteine protease ATG4A |
Synonyms: | Atg4a, ATG4A, APG4A, AUTL2, Autophagy related 4A cysteine peptidase |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse ATG4A |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 45 kDa |
Observed MW. | 48 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | skeletal muscle, pancreas, liver |
Background
Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP.
Images
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Western blot analysis of ATG4 (GB11889) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
