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Anti-ATP5A Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | ATP5A rabbit polyclonal |
Gene Name: | ATP synthase subunit alpha, mitochondrial |
Synonyms: | ATP synthase F1 subunit alpha, Atp5f1a, ATP5A, ATP5A1, ATP5AL2, ATPM, hATP1, MOM2, OMR, ORM |
Immunogen: | Recombinant protein corresponding to Mouse ATP5A |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | |
Predicted MW. | 60 kDa |
Observed MW. | 55 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | kidney, heart |
IHC | Human, Mouse, Rat | 1: 1500-1: 3000 | colon cancer, esophageal cancer, lung cancer, thyroid cancer, colon, esophagus, liver, kidney, stomach, brain, heart, kidney |
Background
The ATP5A1 gene encodes the α subunit of mitochondrial ATP synthase which produces ATP from ADP in the presence of a proton gradient across the membrane. ATP5A1 is localized in the mitochondria and anti-ATP5A1 can be used as the loading control for mitochondrial or Complex V proteins.
Images
| Western blot analysis of ATP5A (GB113455) at dilution of 1: 2000 |
| Immunohistochemistry analysis of paraffin-embedded human colon cancer using ATP5A (GB113455) at dilution of 1: 3000 |
| Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using ATP5A (GB113455) at dilution of 1: 3000 |
| Immunohistochemistry analysis of paraffin-embedded human lung cancer using ATP5A (GB113455) at dilution of 1: 3000 |
| Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using ATP5A (GB113455) at dilution of 1: 3000 |
| Immunohistochemistry analysis of paraffin-embedded human colon using ATP5A (GB113455) at dilution of 1: 3000 |
| Immunohistochemistry analysis of paraffin-embedded human esophagus using ATP5A (GB113455) at dilution of 1: 3000 |
| Immunohistochemistry analysis of paraffin-embedded human liver using ATP5A (GB113455) at dilution of 1: 3000 |
| Immunohistochemistry analysis of paraffin-embedded mouse kidney using ATP5A (GB113455) at dilution of 1: 3000 |
| Immunohistochemistry analysis of paraffin-embedded rat kidney using ATP5A (GB113455) at dilution of 1: 3000 |
| Immunohistochemistry analysis of paraffin-embedded mouse stomach using ATP5A (GB113455) at dilution of 1: 3000 |
| Immunohistochemistry analysis of paraffin-embedded rat stomach using ATP5A (GB113455) at dilution of 1: 3000 |
| Immunohistochemistry analysis of paraffin-embedded rat brain using ATP5A (GB113455) at dilution of 1: 3000 |
| Immunohistochemistry analysis of paraffin-embedded rat heart using ATP5A (GB113455) at dilution of 1: 3000 |
| Immunohistochemistry analysis of paraffin-embedded rat kidney using ATP5A (GB113455) at dilution of 1: 3000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |