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Anti-ATP5L Rabbit pAb (100 μl)
| Reactivity: | M,R & M |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | ATP synthase, H+ transporting, mitochondrial F0 complex, subunit G |
Synonyms: | TP5JG, ATP5L, ATPase subunit g |
Immunogen: | Recombinant protein corresponding to Mouse ATP5L |
Uniprot ID: | Q9CPQ8 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Mitochondrion |
Predicted MW. / Observed MW. | 11 kDa / 12 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 500-1: 1000 | brain, kidney, liver |
IHC/IF | Mouse | 1: 900-1: 1800 | heart |
Background
Mitochondrial membrane ATP synthase (F1-Fo ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. It is composed of the soluble catalytic core, F1, and the membrane-spanning component and Fo, which comprises the proton channel. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP5L gene encodes ATP synthase subunit g of the Fo complex.
Images
| Western blot analysis of ATP5L (GB114132) at dilution of 1: 1000 |
| Immunofluorescence analysis of paraffin embedded mouse heart using ATP5L (GB114132) at dilution of 1: 900 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
