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Anti-ATP6V0D1/P39 Rabbit pAb (100 μl)
| Reactivity: | M,R & H,M,R |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | V-type proton ATPase subunit d 1 |
Synonyms: | V-ATPase subunit d 1, P39, Physophilin, V-ATPase 40 kDa accessory protein, V-ATPase AC39 subunit, Vacuolar proton pump subunit d 1, Atp6v0d1, Atp6d, VPATPD |
Immunogen: | Recombinant protein corresponding to Mouse ATP6V0D1 |
Uniprot ID: | P51863 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 40 kDa / 40 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 500-1: 1000 | cortex, kidney, brain |
ICC/IF | Human, Mouse, Rat | 1: 50-1: 200 | HeLa, NIH-3T3 |
Background
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain.
Images
| Western blot analysis of ATP6V0D1 (GB112113) at dilution of 1: 500 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed HeLa cells using ATP6V0D1(GB112113) at dilution of 1: 50-1: 200 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH-3T3 cells using ATP6V0D1(GB112113) at dilution of 1: 50-1: 200 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
