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Recombinant Anti-Acidic Cytokeratin Mouse mAb (100 μl)
| Reactivity: | H & H |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Keratin, type I cytoskeletal 20 |
Synonyms: | Cytokeratin-20 (CK-20), Keratin-20 (K20), Protein IT |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human CK20 |
Uniprot ID: | P35900 |
Isotype: | IgG1 |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 48 kDa / 40-64 kDa |
Subcellular location: | Cytoplasm |
Product Usage Information
WB | Human | 1: 500-1: 1000 | MCF-7, T-47D, A-431 |
IHC/IF | Human | 1: 500-1: 1000 | colon cancer,esophageal carcinoma |
Background
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.
Images
| WB analysis of Acidic Cytokeratin(GB152051). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 3000, RT, 1h. |
| IHC analysis of Acidic Cytokeratin(GB152051). |
| IHC analysis of Acidic Cytokeratin(GB152051). |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
