Reactivity: | M & M,R |
Applications: | WB & IHC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Gene Name: | THO complex subunit 4 |
Synonyms: | Tho4, Ally of AML-1 and LEF-1, Aly, Ref1, Refbp1, THOC4, Alyref, Transcriptional coactivator Aly/REF, RNA and export factor-binding protein 1, Aly/REF export factor, REF1-I, Aly/Ref |
Immunogen: | Recombinant protein corresponding to Mouse Aly/Ref |
Uniprot ID: | O08583 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Nucleus |
Predicted MW. / Observed MW. | 27 kDa / 34 kDa |
Product Usage Information
WB | Mouse | 1: 500-1: 1000 | heart, spleen, lung, kidney |
IHC/IF | Mouse, Rat | 1: 1000-1: 4000 | heart |
Background
The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins.
Images
Western blot analysis of Aly/Ref (GB111916) at dilution of 1: 1000 | |
Immunohistochemistry analysis of paraffin embedded rat heart using ALYREF (GB111916) at dilution of 1: 2000 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |