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| Reactivity: | M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | BIT1 rabbit polyclonal |
Gene Name: | Peptidyl-tRNA hydrolase 2, mitochondrial |
Synonyms: | BIT1, CGI-147, peptidyl tRNA hydrolase 2, PTH 2, PTH2, PTRH2 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse BIT1 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 20 kDa |
Observed MW. | 18kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human | 1: 300-1: 600 | K562, Raji, HepG2 |
Background
The protein encoded by this gene is a mitochondrial protein with two putative domains, an N-terminal mitochondrial localization sequence, and a UPF0099 domain. In vitro assays suggest that this protein possesses peptidyl-tRNA hydrolase activity, to release the peptidyl moiety from tRNA, thereby preventing the accumulation of dissociated peptidyl-tRNA that could reduce the efficiency of translation. This protein also plays a role regulating cell survival and death. It promotes survival as part of an integrin-signaling pathway for cells attached to the extracellular matrix (ECM), but also promotes apoptosis in cells that have lost their attachment to the ECM, a process called anoikos. After loss of cell attachment to the ECM, this protein is phosphorylated, is released from the mitochondria into the cytosol, and promotes caspase-independent apoptosis through interactions with transcriptional regulators.
Images
| Western blot analysis of BIT1 (GB113740) at dilution of 1: 600 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
