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Anti-CD133 Rabbit pAb (100 μl)
| Reactivity: | H,R & H,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Prominin-1 |
Synonyms: | AC133, Antigen AC133, CD133, CORD12, MCDR2, MSTP061, PROM1, Prominin like protein 1, PROML1, RP41, STGD4, STGD4, Hematopoietic stem cell antigen |
Immunogen: | KLH conjugated Synthetic peptide corresponding to human CD133 |
Uniprot ID: | O43490 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane |
Predicted MW. / Observed MW. | 97kDa / 133kDa |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Rat | 1: 1000-1: 2000 | kidney |
IHC/IF | Human, Rat | 1: 800-1: 1600 | colon cancer, kidney, placenta, pancreas |
Background
CD133, also known as PROM1 (prominin-1) or AC133, belongs to the prominin family. CD133 is a transmembrane glycoprotein with an NH2-terminal extracellular domain, five transmembrane loops and a cytoplasmic tail. The expression of CD133 has been reported in hematopoietic stem cells, endothelial progenitor cells, neuronal and glial stem cells, suggesting the potential role of CD133 as a cell surface marker of adult stem cells. CD133 has also been reported as a marker of cancer stem cells in various human tumors.
Images
| Western blot analysis of CD133 (GB114579) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded human colon cancer using CD133 (GB114579) at dilution of 1: 800 |
| Immunohistochemistry analysis of paraffin embedded human kidney using CD133 (GB114579) at dilution of 1: 800 |
| Immunohistochemistry analysis of paraffin embedded human pancreas using CD133 (GB114579) at dilution of 1: 800 |
| Immunohistochemistry analysis of paraffin embedded human renal carcinoma using CD133 (GB114579) at dilution of 1: 800 |
| Immunohistochemistry analysis of paraffin embedded rat kidney using CD133 (GB114579) at dilution of 1: 800 |
| Immunohistochemistry analysis of paraffin embedded rat pancreas using CD133 (GB114579) at dilution of 1: 800 |
| Immunohistochemistry analysis of paraffin embedded rat placenta using CD133 (GB114579) at dilution of 1: 800 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
