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| Reactivity: | M,R |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | CD14 rabbit polyclonal antibody |
Gene Name: | Monocyte differentiation antigen CD14 |
Synonyms: | CD14, entrez:929, CD14 molecule |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse CD14 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane, Secreted, Membrane raft, Golgi apparatus |
Predicted MW. | 40 kDa |
Observed MW. | 45 kDa |
Uniprot ID: | P08571, P10810, Q63691 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | spleen, thymus, lymph node |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | lung, pneumonia, tonsil |
Background
Coreceptor for bacterial lipopolysaccharide . In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) . Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response . Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL-.
Images
| Western blot analysis of CD14 (GB11254) at dilution of 1: 500 |
| Immunohistochemistry of paraffin embedded human tonsil using CD14 (GB11254) at dilution of 1: 500 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse lung using CD14 (GB11254) at dilution of 1: 500 (400x lens) |
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Immunohistochemistry of paraffin embedded rat pneumonia using CD14 (GB11254) at dilution of 1: 500 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
